JCM 5046 PDF

Gorisar However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only effective tools for preventing the acquisition and spread of new carbapenemase-encoding genes. Please review our policy. Jck, we performed transformation by electroporation into Escherichia coli Top Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates.

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Nelmaran A total of Klebsiella strains collected from previous surveillance studies were randomly selected for this study, in order to represent distinct Brazilian geographic regions.

Journal List Antimicrob Jcn Chemother v. Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ.

In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only hcm tools for preventing the acquisition and spread of new carbapenemase-encoding genes. Only 2 BKCproducing K. Antimicrobial susceptibility profiles of BKCproducing K. Support Center Support Center. Prepublished online May To date, inactivation of mgrB has been the main mechanism of polymyxin resistance observed in K. Author information Article notes Copyright and License information Disclaimer.

This article has been cited by other articles in PMC. S, sensitive; R, resistant. Результаты поиска для JCM The other authors have no conflicts to declare. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard— 10th ed CLSI document MA Only 2 of Klebsiella isolates 0.

Clinical and Laboratory Standards Institute. Plasmid extraction of K. Transformant cells were selected on LB agar plates supplemented with 0. The Kpn and KpnJ strains showed resistance to all beta-lactams and fluoroquinolones. National Center for Biotechnology InformationU.

Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype. Please review our privacy policy. The bla BKC-1 gene was inserted into a kb plasmid that was identical to the previously jc, plasmid, p Performance standards for antimicrobial susceptibility testing; 25th informational supplement. Initially, we performed transformation by electroporation into Escherichia coli Top Kpn showed susceptibility to tigecycline MIC, 0.

The Kpn and KpnJ strains showed different plasmid profiles. Related Posts

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JCM 5046 PDF

Bazahn Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. Open in a separate window. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8. Santosb Jorge L. The carbapenemase was encoded by bla BKC-1which was located on a small nonconjugative plasmid 2.

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Nelmaran A total of Klebsiella strains collected from previous surveillance studies were randomly selected for this study, in order to represent distinct Brazilian geographic regions. Journal List Antimicrob Jcn Chemother v. Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only hcm tools for preventing the acquisition and spread of new carbapenemase-encoding genes. Only 2 BKCproducing K.

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Martinsa Adriana G. BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Author information Article notes Copyright and License information Disclaimer. The two BKCproducing isolates belonged to clonal complex and possessed identical pulsed-field gel electrophoresis patterns.

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This article has been cited by other articles in PMC. However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. Frequency of BKCproducing Ucm species isolates. These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

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